Western blot analysis was performed on whole cell extracts of HeLa (Lane 1), MDA-MB-231 (Lane 2), Daudi (Lane 3), Raji (Lane 4) and Ramos (Lane 5). The blot was probed with Anti-Vimentin Antibody Cocktail (Product # MA5-14435, 100 ul) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution), Goat anti-Mouse IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4,000 dilution) and Rabbit anti-Goat IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/mL, 1:4,000 dilution) as the host species was mixed category. A 55 kDa band corresponding to Vimentin was observed across the cell lines tested except in the cell lines of the B-lymphocyte class.
Formalin-fixed paraffin-embedded human melanoma stained with the MultiVision anti-Mouse/ HRP + anti-Rabbit/AP. Note: Cytoplasmic staining of Vimentin (in blue) and gp100 (in red).
Supplier Page from Thermo Fisher Scientific for Vimentin Antibody